Biotechnology:

Tools and Techniques

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Agarose Gel Demos

Agarose Gel Demos

Watch a "how-to" video on the proper technique of preparing an agarose gel for use in gel electrophoresis in this video segment from Austin Community College. A supplementary video shows the proper technique for pouring an agarose gel.

9-12

Video

DNA Extraction

DNA Extraction

This interactive activity adapted from the University of Nebraska's Plant and Soil Sciences eLibrary illustrates how DNA, which is normally locked inside of cells, is extracted from living tissue. OER Level

9-12

Interactive

DNA Libraries

DNA Libraries

This interactive resource adapted from the Dolan DNA Learning Center explores the different types of DNA libraries used to store and access genetic information. OER Level

9-12

Interactive

DNA Separation Lab in the Classroom and in Real-World Research

DNA Separation Lab in the Classroom and in Real-World Research

This video produced by WGBH features students in the Amgen-Bruce Wallace Biotechnology Lab Program preparing a recombinant DNA molecule, and examines how this work relates to the way real-life scientists approach similar work. OER Level

9-12

Video

DNA Sequencing

DNA Sequencing

This interactive activity adapted from the Dolan DNA Learning Center looks at two techniques used to sequence DNA: the Sanger method and an automated process based on the Sanger method. OER Level

9-12

Interactive

Electrophoresis and Gel Analysis

Electrophoresis and Gel Analysis

In this animation produced by WGBH and Digizyme, Inc., see how molecules of DNA are separated using gel electrophoresis, and how this process enables scientists to compare the molecular variations of two or more DNA samples. OER Level

9-12

Video

Gel Electrophoresis

Gel Electrophoresis

This interactive activity from the Dolan DNA Learning Center illustrates the process of gel electrophoresis, in which DNA fragments are separated by size as they migrate at different rates through a gel matrix. OER Level

6-12

Interactive

Gene Cloning

Gene Cloning

This interactive activity adapted from the University of Nebraska's Library of Crop Technologies details the steps involved in producing clones of genes that can then be used to transform the characteristics of an organism. OER Level

9-12

Interactive

Genetically Engineering the Avian Flu

Genetically Engineering the Avian Flu

In this video segment adapted from NOVA scienceNOW, learn about a radical biotechnology experiment aimed at figuring out how to stop the avian flu virus. OER Level

9-12

Video

Genetic Engineering and Working with DNA

Genetic Engineering and Working with DNA

In this animation produced by WGBH and Digizyme, Inc., see how bacterial plasmids are engineered to clone or express genes that code for proteins such as human insulin. OER Level

9-12

Video

Growing Bacterial Cultures

Growing Bacterial Cultures

This animation produced by WGBH and Digizyme, Inc. demonstrates the techniques researchers use to isolate transformed bacteria and expand their population in order to obtain large quantities of a protein of interest. OER Level

9-12

Video

Longevity Genes

Longevity Genes

What is the secret to a long life? Hear from scientists using biotechnology to analyze relationships between specific genes and longevity in this video segment adapted from NOVA scienceNOW. OER Level

9-12

Video

Making an Agarose Gel

Making an Agarose Gel

This video adapted from the University of Leicester demonstrates the step-by-step process of making and pouring an agarose gel, a key component in gel electrophoresis. OER Level

9-12

Video

Model Organisms

Model Organisms

This interactive activity from the Dolan DNA Learning Center shows the different types of model organisms used in research and why each might be selected when performing an experiment. OER Level

9-12

Interactive

Pipetting Demos

Pipetting Demos

Watch two "how-to" videos on the proper technique of pipetting in this video segment from Austin Community College.

9-12

Video

Polymerase Chain Reaction

Polymerase Chain Reaction

This interactive activity adapted from the University of Nebraska's Library of Crop Technologies depicts steps in the polymerase chain reaction (PCR) technique and explains how it is used to efficiently copy sections of DNA for analysis. OER Level

9-12

Interactive

Protein Purification

Protein Purification

This animation produced by WGBH and Digizyme, Inc. demonstrates how a protein of interest is isolated from other contents in a transformed bacterial cell—a process called purification—using a lab technique called hydrophobic interaction chromatography. OER Level

9-12

Video

Protein Purification Lab in the Classroom and in Real-World Research

Protein Purification Lab in the Classroom and in Real-World Research

This video produced by WGBH features students in the Amgen-Bruce Wallace Biotechnology Lab Program purifying a protein, and also examines the motivations people have for choosing a career in science. OER Level

9-12

Video

Recombinant DNA Lab in the Classroom and in Real-World Research

Recombinant DNA Lab in the Classroom and in Real-World Research

This video produced by WGBH features students in the Amgen-Bruce Wallace Biotechnology Lab Program inserting a recombinant DNA molecule into bacterial cells, and illustrates how industry and academic scientists use the exact same techniques. OER Level

9-12

Video

Running an Agarose Gel

Running an Agarose Gel

This video adapted from the University of Leicester provides step-by-step instructions for loading samples into an agarose gel and then running the gel to separate DNA molecules according to their size. OER Level

9-12

Video

Transforming Bacteria

Transforming Bacteria

This animation produced by WGBH and Digizyme, Inc. demonstrates the experimental technique used to introduce DNA plasmids inside bacterial cells, a process called bacterial transformation. OER Level

9-12

Video

Using a Micropipette

Using a Micropipette

This video adapted from the University of Leicester demonstrates the proper use of different-sized micropipettes to measure precise volumes of liquid in the lab. OER Level

9-12

Video

Working with Small Volumes

Working with Small Volumes

This video from BenchFly.com demonstrates a lab technique that can be helpful when pipetting very small volumes of liquid. OER Level

9-12

Video